Liu Min, Li Xiaolin, Bie Wei, Wang Minglin, Feng Qian
College of Food Science and Engineering, Shandong Agricultural University, Taian 271000, China.
Se Pu. 2011 Feb;29(2):162-7. doi: 10.3724/sp.j.1123.2011.00162.
A new method was established for the determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography (SPE-HPLC). The samples were extracted by methanol-water (1:1, v/v) and purified by a solid phase extraction column. Then, the chromatographic separation was achieved on a Luna C18 column by linear gradient elution. The mobile phase was 10 mmol/L ammonium acetate-acetonitrile (containing 1% acetic acid). The results showed that the 15 industrial synthetic dyes can be separated efficiently. The recoveries of the 15 industrial synthetic dyes spiked in condiment were between 84.6% and 114.2% with the relative standard deviations of 0.9% - 10.3%. The limits of detection of this method was 0.05 - 0.18 mg/kg for the 15 industrial synthetic dyes. The method is simple, sensitive, accurate, repeatable and can be used for simultaneous determination of the 15 illegally added industrial synthetic dyes.
建立了一种固相萃取-高效液相色谱法（SPE-HPLC）测定调味品中15种工业合成染料的新方法。样品用甲醇-水（1:1，v/v）提取，并用固相萃取柱净化。然后，在Luna C18柱上通过线性梯度洗脱进行色谱分离。流动相为10 mmol/L醋酸铵-乙腈（含1%醋酸）。结果表明，15种工业合成染料能够有效分离。在调味品中添加的15种工业合成染料的回收率在84.6%至114.2%之间，相对标准偏差为0.9% - 10.3%。该方法对15种工业合成染料的检出限为0.05 - 0.18 mg/kg。该方法简便、灵敏、准确、可重复，可用于同时测定15种非法添加的工业合成染料。
