Zhang Juzhou, Li Jing, Shao Dongliang, Yao Bangben, Jiang Junshu
Anhui National Center of Agricultural Standardization and Supervision, China National Center for Quality Supervision and Test of Agricultural-Avocation Processed Food, Hefei 230051, China.
Se Pu. 2012 Feb;30(2):190-5. doi: 10.3724/sp.j.1123.2011.10002.
An effective high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of 9 ultraviolet stabilizers in food plastic packaging materials. The food packaging samples were firstly extracted by methanol-ethyl acetate, and then purified by a C18 solid-phase extraction (SPE) column. The target compounds were separated on a ZORBAX SB-C18 column (250 mm x 4.6 mm, 5 microm) in gradient elution mode using methanol and water as mobile phases. The detection wavelength was at 310 nm. The linear plots of the nine ultraviolet stabilizers were obtained between 0.2 and 10 mg/L, with the correlation coefficients of above 0. 999 for the nine ultraviolet stabilizers. The limits of detection for this method were in the range from 0.05 to 0.1 mg/L. The recoveries spiked in commercial food plastic packaging materials were in the range of 70.2% - 89.0% with the relative standard deviations of 0.4% - 4.5%. The results indicated that the method is simple, accurate, and suitable for the simultaneous determination of the nine ultraviolet stabilizers in food plastic packaging materials.
已开发出一种高效液相色谱（HPLC）方法，用于同时测定食品塑料包装材料中的9种紫外线稳定剂。食品包装样品首先用甲醇 - 乙酸乙酯萃取，然后通过C18固相萃取（SPE）柱进行净化。目标化合物在ZORBAX SB - C18柱（250 mm×4.6 mm，5μm）上以梯度洗脱模式分离，使用甲醇和水作为流动相。检测波长为310 nm。9种紫外线稳定剂的线性范围为0.2至10 mg/L，9种紫外线稳定剂的相关系数均高于0.999。该方法的检测限在0.05至0.1 mg/L范围内。在商业食品塑料包装材料中加标的回收率在70.2% - 89.0%之间，相对标准偏差为0.4% - 4.5%。结果表明，该方法简便、准确，适用于同时测定食品塑料包装材料中的9种紫外线稳定剂。
