Chen Dong-Yang, Zhang Hao, Feng Jia-Li, Zeng Dong, Ding Li, Liu Xian-Jun, Li Bang-Rui
Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, Hunan, China.
J Chromatogr Sci. 2017 Nov 1;55(10):1021-1025. doi: 10.1093/chromsci/bmx070.
An efficient method was developed for the simultaneous determination of 10 industrial dyes (basic orange 2, basic orange 21, basic orange 22, acid orange II, auramine, basic rhodamine B and Sudan I-IV) in the foodstuffs using high-performance liquid chromatography coupled with diode array detector. Samples were extracted with acetonitrile and cleaned up on a solid phase extraction cartridge using HLB. The chromatographic separation was achieved on a C18 column using a mobile phase consisting of methanol and 10 mmol/L ammonium acetate with 0.1% formic acid by gradient elution. Good linearity (r ≥ 0.9993) was observed between 0.050 and 5.0 μg/mL. The limits of detection were in the range of 0.007-0.01 mg/kg, high recoveries (80.6-104%) and good reproducibility (1.1-5.7%) were obtained. Such method is simple, feasible and accurate, which can be applied to the quantification of 10 dyes in food samples.
建立了一种高效方法，采用高效液相色谱-二极管阵列检测器同时测定食品中的10种工业染料（碱性橙2、碱性橙21、碱性橙22、酸性橙II、金胺、碱性罗丹明B和苏丹红I-IV）。样品用乙腈提取，并用HLB固相萃取柱净化。在C18柱上进行色谱分离，流动相由甲醇和10 mmol/L醋酸铵与0.1%甲酸组成，采用梯度洗脱。在0.050至5.0 μg/mL之间观察到良好的线性关系（r≥0.9993）。检测限在0.007-0.01 mg/kg范围内，回收率高（80.6-104%），重现性好（1.1-5.7%）。该方法简单、可行、准确，可用于食品样品中10种染料的定量分析。
