Münstermann U, Fritz G, Seitz G, Lu Y P, Schneider H R, Issinger O G
Institut für Humangenetik, Universität des Saarlandes, Homburg, Federal Republic of Germany.
Eur J Biochem. 1990 Apr 30;189(2):251-7. doi: 10.1111/j.1432-1033.1990.tb15484.x.
Protein kinase CKII (i.e. casein kinase II, CKII, NII) is expressed at a higher level in rapidly proliferating tissues and in solid human tumours (e.g. colorectal carcinomas) when compared to the corresponding non-neoplastic colorectal mucosa. This could be shown by (a) Western blotting of cellular extracts from solid tumours followed by immunostaining with an anti-CKII polyclonal antibody, (b) immunohistochemical staining of cells from tissue sections and (c) by activity measurements using the CKII-specific synthetic peptide (RRRDDDSDDD). The maximum observed activity in the colorectal carcinomas investigated was up to eightfold higher in the tumour specimens than in the non-neoplastic tissue (i.e. colorectal mucosa). The activity range was between 33-350 U/mg protein and in the case of colorectal mucosa 13-106 U/mg protein. The amount of CKII determined in the individual tumours was in the range 0.4-1.6 nmol/g tissue.
蛋白激酶CKII（即酪蛋白激酶II，CKII，NII）在快速增殖组织和人类实体瘤（如结直肠癌）中的表达水平高于相应的非肿瘤性结直肠黏膜。这可以通过以下方法证明：（a）对实体瘤细胞提取物进行蛋白质免疫印迹，然后用抗CKII多克隆抗体进行免疫染色；（b）对组织切片中的细胞进行免疫组织化学染色；（c）使用CKII特异性合成肽（RRRDDDSDDD）进行活性测量。在所研究的结直肠癌中，观察到的肿瘤标本中的最大活性比非肿瘤组织（即结直肠黏膜）高八倍。活性范围在33 - 350 U/mg蛋白质之间，结直肠黏膜的活性范围为13 - 106 U/mg蛋白质。在各个肿瘤中测定的CKII量在0.4 - 1.6 nmol/g组织范围内。
