Prowald K, Fischer H, Issinger O G
FEBS Lett. 1984 Oct 29;176(2):479-83. doi: 10.1016/0014-5793(84)81222-3.
Casein kinase II (CKII) activity is enhanced as much as 2-3 fold in established and 4-5-fold in transformed human cell lines when compared to that of fibroblasts and primary human tumour cell cultures where CKII activity never exceeded a basic level. The high activity of CKII in transformed cells and in established cell lines was reduced to about the same basic level after treatment with heparin, a highly specific inhibitor of CKII activity. The activity of the cAMP-dependent protein kinase was virtually the same in fibroblasts and various human tumour cell lines investigated.
与成纤维细胞和原代人肿瘤细胞培养物相比，酪蛋白激酶II（CKII）在已建立的人细胞系中的活性增强了2至3倍，在转化的人细胞系中增强了4至5倍，而成纤维细胞和原代人肿瘤细胞培养物中的CKII活性从未超过基础水平。在用肝素（一种高度特异性的CKII活性抑制剂）处理后，转化细胞和已建立细胞系中CKII的高活性降低至大致相同的基础水平。在研究的成纤维细胞和各种人肿瘤细胞系中，cAMP依赖性蛋白激酶的活性实际上是相同的。
