Department of Pharmaceutics, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, 3584 CG, Utrecht, The Netherlands.
J Control Release. 2011 Sep 25;154(3):218-32. doi: 10.1016/j.jconrel.2011.05.001. Epub 2011 May 7.
Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field.
筛选新的基因传递候选物的转染效率和毒性，通常是通过体外转染后相对参考制剂读出转基因表达水平来进行的。然而，多年来，不同的实验室在各种细胞系中，使用各种条件和读出系统，并与各种参考制剂进行了筛选。这使得结果的直接比较变得困难，如果不是不可能的话。达成共识将使新的结果能够融入以前的发现的背景，并估计对非病毒基因传递的改进的总体贡献。在本文中，我们说明了所选测试条件对转染结果的敏感性，并提出了一个筛选方案，旨在实现该领域的标准化。
