Erciyes University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Kayseri, Turkey.
Food Chem Toxicol. 2011 Sep;49(9):1960-4. doi: 10.1016/j.fct.2011.05.006. Epub 2011 May 11.
The present study was aimed at the investigation of the antioxidative effect of evening primrose oil in cases of subacute aflatoxin (AF) intoxication induced in mice. For this purpose, forty-eight 6-8-week-old male BALB/c mice, weighing 30-35 g, were used. The animals were allocated to four groups, each comprising of 12 mice, such that one group was maintained as the control group and the other three constituted the trial groups. The mice included in the control group (Group 1) were not subjected to any treatment. Group 2 was administered with 1.5 ml/kg bw/day of evening primrose oil; Group 3 received 1250 μg/kg bw/day of AF (82.45% AFB(1), 10.65% AFB(2), 4.13% AFG(1) and 2.77% AFG(2)) and Group 4 was given 1250 μg/kg bw/day of AF plus 1.5 ml/kg bw/day of evening primrose oil using a catheter, for a period of 14 days. At the end of the 14th day, the liver, lungs, kidneys, brain, heart and spleen of the animals included in all groups were extracted. Malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidise (GSH-Px) activities were measured in tissue homogenates. In result, it was concluded that, evening primrose oil had a positive effect on aflatoxin-induced lipid peroxidation.
本研究旨在探讨月见草油对亚急性黄曲霉毒素（AF）中毒的抗氧化作用。为此，使用了 48 只 6-8 周龄、体重 30-35g 的雄性 BALB/c 小鼠，将它们分为四组，每组 12 只，其中一组作为对照组，另外三组为试验组。对照组（第 1 组）的小鼠未进行任何处理。第 2 组给予 1.5ml/kg bw/天的月见草油；第 3 组给予 1250μg/kg bw/天的 AF（82.45%AFB(1)、10.65%AFB(2)、4.13%AFG(1)和 2.77%AFG(2))；第 4 组通过导管给予 1250μg/kg bw/天的 AF 加 1.5ml/kg bw/天的月见草油，为期 14 天。第 14 天结束时，提取所有组动物的肝、肺、肾、脑、心和脾。在组织匀浆中测量丙二醛（MDA）水平以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。结果表明，月见草油对黄曲霉毒素诱导的脂质过氧化具有积极作用。
