Erciyes University, Safiye Çıkrıkçıoğlu Vocational College, Department of Animal Science, Kayseri, Turkey.
Ecotoxicol Environ Saf. 2011 Oct;74(7):2119-25. doi: 10.1016/j.ecoenv.2011.07.002. Epub 2011 Aug 17.
Forty-eight male Balb/C mice, allocated to 4 equal groups, constituted the material of the study. The first group was maintained as the control group and was administered solely with a vehicle, which was used to dissolve coumaphos in the third and fourth groups. The second group was administered with 1.5 ml/kg.bw/day (∼1400 mg/kg.bw/day) of wheat germ oil. The third group received 5.5mg/kg.bw/day (1/10 LD50(oral)) of coumaphos. Finally, the fourth group was given both coumaphos and wheat germ oil at the doses indicated above. In all groups, the compounds were given directly into the stomach using a gastric tube, and treatment was continued for a period of 45 days. At the end of the 45th day, the liver, lungs, kidneys, brain, heart and spleen were extirpated in all of the animals. Tissue homogenates prepared from the tissue specimens were analysed for malondialdehyde (MDA) levels and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. In conclusion, it was determined that coumaphos led to adverse alterations in the majority of the oxidative stress markers investigated. The administration of wheat germ oil alleviated the coumpahos-induced adverse effects detected in the tissues examined.
四十八只雄性 Balb/C 小鼠，平均分配到 4 组，构成了研究的材料。第一组作为对照组，仅给予溶剂，该溶剂用于溶解第三和第四组中的氯菊酯。第二组给予 1.5ml/kg.bw/天（约 1400mg/kg.bw/天）的小麦胚芽油。第三组给予 5.5mg/kg.bw/天（1/10LD50（口服））的氯菊酯。最后，第四组同时给予上述剂量的氯菊酯和小麦胚芽油。在所有组中，通过胃管直接将化合物给予胃中，治疗持续 45 天。在第 45 天结束时，从所有动物中取出肝脏、肺、肾、脑、心脏和脾脏。从组织标本制备的组织匀浆用于分析丙二醛（MDA）水平以及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。总之，确定氯菊酯导致大多数研究的氧化应激标志物发生不良改变。小麦胚芽油的给予缓解了在检查的组织中检测到的氯菊酯引起的不良影响。
