Seroogy K B, Mohapatra N K, Lund P K, Réthelyi M, McGehee D S, Perl E R
Department of Physiology, University of North Carolina, Chapel Hill 27599.
Brain Res Mol Brain Res. 1990 Feb;7(2):171-6. doi: 10.1016/0169-328x(90)90095-u.
The expression of cholecystokinin (CCK) messenger RNA (mRNA) was examined in dorsal root ganglia of rat and guinea pig using in situ hybridization histochemistry and RNA (Northern) blot hybridization with synthetic oligodeoxyribonucleotide (oligomer) probes. In guinea pig, CCK mRNA was detected in small and medium-sized neuronal perikarya comprising approximately 10-15% of the total dorsal root ganglia cell population. In contrast, in neurons of rat dorsal root ganglia, CCK mRNA was not detectable. Northern blot analyses revealed a single CCK mRNA species of expected size (0.8 kb) in guinea pig, but not rat, dorsal root ganglia. A 0.8 kb CCK mRNA was, however, detected in cortex of both rat and guinea pig. These data suggest that CCK is normally not synthesized in neurons of rat dorsal root ganglia and that there are species differences in CCK gene expression in mammalian sensory ganglia.
采用原位杂交组织化学和RNA（Northern）印迹杂交技术，使用合成的寡脱氧核糖核苷酸（寡聚物）探针，检测大鼠和豚鼠背根神经节中胆囊收缩素（CCK）信使核糖核酸（mRNA）的表达。在豚鼠中，在中小型神经元胞体中检测到CCK mRNA，这些胞体约占背根神经节细胞总数的10%-15%。相比之下，在大鼠背根神经节神经元中，未检测到CCK mRNA。Northern印迹分析显示，在豚鼠而非大鼠的背根神经节中，有一个预期大小（0.8 kb）的单一CCK mRNA种类。然而，在大鼠和豚鼠的皮质中均检测到0.8 kb的CCK mRNA。这些数据表明，CCK通常不在大鼠背根神经节神经元中合成，并且在哺乳动物感觉神经节中CCK基因表达存在种属差异。
