Sutin E L, Jacobowitz D M
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892.
Brain Res Mol Brain Res. 1990 Jun;8(1):63-8. doi: 10.1016/0169-328x(90)90010-b.
The regional distribution of dorsal pontine tegmental neurons expressing cholecystokinin (CCK) messenger RNA (mRNA) was examined using in situ hybridization histochemical techniques. A distribution of neurons containing CCK mRNA was observed in the motor nucleus of the trigeminal nerve, a region which has not been shown to contain CCK by immunocytochemical means. In addition, CCK mRNA was seen in the caudal portion of the dorsal raphe nucleus which correlates with prior immunocytochemical observations. The presence of CCK mRNA in the cholinergic motor trigeminal nucleus suggests that these cells express the CCK gene and have gone undetected in previous immunocytochemical studies. It is also suggested that CCK may have an influence on muscular contraction at sites of trigeminal nerve innervation (e.g. muscles of mastication).
采用原位杂交组织化学技术，研究了表达胆囊收缩素（CCK）信使核糖核酸（mRNA）的脑桥背侧被盖神经元的区域分布。在三叉神经运动核中观察到含有CCK mRNA的神经元分布，而通过免疫细胞化学方法尚未发现该区域含有CCK。此外，在中缝背核尾部可见CCK mRNA，这与先前的免疫细胞化学观察结果相关。胆碱能运动三叉神经核中存在CCK mRNA表明这些细胞表达CCK基因，且在先前的免疫细胞化学研究中未被发现。还表明CCK可能对三叉神经支配部位（如咀嚼肌）的肌肉收缩有影响。
