黑腹果蝇培养细胞系中蜕皮甾体受体的证明。
Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.
作者信息
Maroy P, Dennis R, Beckers C, Sage B A, O'Connor J D
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6035-8. doi: 10.1073/pnas.75.12.6035.
Abstract
Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 X 10(-9) M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 X 10(-10) M which probably reflects the molar concentration of cytoplasmic receptors.
摘要
用[3H]蜕皮甾酮A孵育黑腹果蝇Kc细胞系的高速上清液，通过凝胶过滤和葡聚糖包被活性炭结合试验测定，结果显示配体有显著结合。Kc细胞系在体内用[3H]蜕皮甾酮A处理30分钟，结果KCl可溶性核提取物能显著结合该配体。对于胞质溶胶和细胞核制剂，这种结合都具有特异性且解离常数较低（3×10^(-9) M）。此外，细胞核制剂的标记在大约7×10^(-10) M时呈现饱和，这可能反映了细胞质受体的摩尔浓度。
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