黑腹果蝇Kc细胞系对蜕皮甾酮A的摄取与释放

The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster.

作者信息

Beckers C, Maróy P, Dennis R, O'Connor J D, Emmerich H

出版信息

Mol Cell Endocrinol. 1980 Jan;17(1):51-9. doi: 10.1016/0303-7207(80)90103-3.

DOI:10.1016/0303-7207(80)90103-3

Abstract

The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with their biological activity on this Kc cell line, the physiological activity ratio for PNA, 20-hydroxyecdysone and ecdysone is 1 : 50 : 2000, resp. Both association and release of [3H]-PNA are temperature-dependent, the activation energy was calculated as 16.7 cal (Arrhenius analysis). This association is compatible with unlabelled PNA and various ecdysteroids. The KD for PNA (Scatchard analysis) was estimated as 3.6 x 10(-9) M, giving the number of binding sites as approx. 1800 per cell.

摘要

蜕皮甾酮A（PNA）和20-羟基蜕皮激素与Kc细胞的结合与其对该Kc细胞系的生物活性相称，PNA、20-羟基蜕皮激素和蜕皮激素的生理活性比分别为1:50:2000。[3H]-PNA的结合和释放均与温度有关，通过阿累尼乌斯分析计算出活化能为16.7卡。这种结合与未标记的PNA和各种蜕皮甾体相容。通过斯卡特查德分析估计PNA的解离常数（KD）为3.6×10^(-9) M，得出每个细胞的结合位点数约为1800个。

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