Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
Exp Gerontol. 2011 Sep;46(9):768-72. doi: 10.1016/j.exger.2011.05.002. Epub 2011 May 13.
In the present study, we compared HSP90 and its co-chaperone, p23, immunoreactivity and their protein levels in the hippocampus between adult (postnatal month 6) and aged (postnatal month 24) gerbils using immunohistochemistry and western blot analysis. HSP90 immunoreactivity was markedly increased in pyramidal cells in the hippocampus proper and in polymorphic cells in the dentate gyrus of the aged group compared to the adult group. p23 immunoreactivity was slightly increased in pyramidal cells of the hippocampus proper and in granule cells of the dentate gyrus in the aged group. In addition, HSP90 and p23 protein levels in the aged hippocampus were much higher than the adult hippocampus. These results indicate that HSP90 and p23 immunoreactivity and protein levels in the hippocampus are distinctively increased in the aged gerbils compared to the adult gerbils.
在本研究中，我们通过免疫组织化学和 Western blot 分析比较了成年（出生后 6 个月）和老年（出生后 24 个月）沙鼠海马中的 HSP90 及其共伴侣 p23 的免疫反应性和蛋白水平。与成年组相比，老年组的海马中锥体神经元和齿状回多形细胞中的 HSP90 免疫反应性明显增加。p23 免疫反应性在老年组的海马中锥体神经元和齿状回颗粒细胞中略有增加。此外，老年海马中的 HSP90 和 p23 蛋白水平明显高于成年海马。这些结果表明，与成年沙鼠相比，老年沙鼠海马中的 HSP90 和 p23 免疫反应性和蛋白水平明显增加。
