Comer J A, Tesh R B, Modi G B, Corn J L, Nettles V F
College of Veterinary Medicine, University of Georgia, Athens.
Am J Trop Med Hyg. 1990 May;42(5):483-90. doi: 10.4269/ajtmh.1990.42.483.
Laboratory-reared female sand flies (Lutzomyia shannoni) were experimentally infected, orally and by intrathoracic inoculation, with the New Jersey serotype of vesicular stomatitis (VSNJ) virus. Virus replication occurred in the insects following infection by both routes. Virus titers greater than 10(4) plaque forming units of VSNJ virus were present in heads of orally infected sand flies 12 days after virus ingestion, confirming that a persistent disseminated infection had occurred. Both orally and parenterally infected Lu. shannoni transmitted VSNJ virus by bite to susceptible rodents and by transovarial transmission to a small percentage of their F1 progeny. The significance of these findings in the epizootiology of VSNJ virus on Ossabaw Island, Georgia, an enzootic focus of this virus, is discussed.
在实验室饲养的雌性沙蝇（Lutzomyia shannoni）通过口服和胸腔内接种的方式，被新泽西血清型水疱性口炎（VSNJ）病毒实验性感染。两种感染途径后，病毒均在昆虫体内复制。在摄入病毒12天后，口服感染的沙蝇头部存在滴度大于10(4) 个VSNJ病毒蚀斑形成单位的病毒，证实发生了持续性播散感染。口服和经肠外途径感染的L. shannoni均通过叮咬将VSNJ病毒传播给易感啮齿动物，并通过经卵传递将病毒传播给一小部分F1代子代。讨论了这些发现在佐治亚州奥萨巴岛VSNJ病毒动物流行病学中的意义，该岛是这种病毒的一个地方病疫源地。
