MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
J Bacteriol. 2011 Jul;193(14):3482-9. doi: 10.1128/JB.05010-11. Epub 2011 May 20.
Heterocysts are specialized cells required for aerobic fixation of dinitrogen by certain filamentous cyanobacteria. Numerous genes involved in the differentiation and function of heterocysts in Anabaena sp. strain PCC 7120 have been identified by mutagenizing and screening for mutants that require fixed nitrogen for growth in the presence of oxygen. We have verified that 10 Anabaena sp. genes, all1338, all1591, alr1728, all3278, all3520, all3582, all3850, all4019, alr4311, and all4388, identified initially by transposon mutagenesis, are such genes by complementing or reconstructing the original mutation and by determining whether the mutant phenotype might be due to a polar effect of the transposon. Elucidation of the roles of these genes should enhance understanding of heterocyst biology.
异形胞是某些丝状蓝藻进行有氧固氮所必需的特化细胞。通过诱变和筛选需要在氧气存在下固定氮生长的突变体，已经鉴定出参与鱼腥藻 PCC 7120 异形胞分化和功能的许多基因。我们已经验证了最初通过转座子诱变鉴定的 10 个鱼腥藻基因，all1338、all1591、alr1728、all3278、all3520、all3582、all3850、all4019、alr4311 和 all4388，通过互补或重建原始突变以及确定突变表型是否可能是转座子的极性效应所致，这些基因都是这样的基因。阐明这些基因的作用应该会增强对异形胞生物学的理解。
