蓝藻鱼腥藻PCC 7120中第2组σ因子SigC对异形胞分化相关基因的调控
Regulation of Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120 by a Group 2 Sigma Factor SigC.
作者信息
Ehira Shigeki, Miyazaki Shogo
机构信息
Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan.
Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
出版信息
Life (Basel). 2015 Feb 16;5(1):587-603. doi: 10.3390/life5010587.
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 differentiates specialized cells for nitrogen fixation called heterocysts upon limitation of combined nitrogen in the medium. During heterocyst differentiation, expression of approximately 500 genes is upregulated with spatiotemporal regulation. In the present study, we investigated the functions of sigma factors of RNA polymerase in the regulation of heterocyst differentiation. The transcript levels of sigC, sigE, and sigG were increased during heterocyst differentiation, while expression of sigJ was downregulated. We carried out DNA microarray analysis to identify genes regulated by SigC, SigE, and SigG. It was indicated that SigC regulated the expression of genes involved in heterocyst differentiation and functions. Moreover, genes regulated by SigC partially overlapped with those regulated by SigE, and deficiency of SigC was likely to be compensated by SigE.
丝状蓝细菌鱼腥藻Anabaena sp. PCC 7120菌株在培养基中化合态氮受限的情况下，会分化出用于固氮的特化细胞，即异形胞。在异形胞分化过程中，约500个基因的表达会通过时空调控上调。在本研究中，我们研究了RNA聚合酶的σ因子在异形胞分化调控中的功能。sigC、sigE和sigG的转录水平在异形胞分化过程中升高，而sigJ的表达下调。我们进行了DNA微阵列分析，以鉴定受SigC、SigE和SigG调控的基因。结果表明，SigC调控参与异形胞分化和功能的基因表达。此外，受SigC调控的基因与受SigE调控的基因部分重叠，SigC的缺失可能由SigE补偿。
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