Research Institute for Oncology-IPON, Federal University of the Triângulo Mineiro-UFTM, Uberaba, Minas Gerais, Brazil.
Clin Med Insights Oncol. 2011;5:107-15. doi: 10.4137/CMO.S6927. Epub 2011 Apr 25.
Cancer stems from mutations in specific genes that induce uncontrolled cell proliferation. Dendritic cells (DCs) are important immunologic cells and play a crucial role in the induction of an antitumour response.
We examined the immune response mediated by T lymphocytes, helper T cells, cytotoxic T cells, and regulatory T cells, as well as the cytokines [interleukin (IL)-2, IL-12, interferon (IFN)-γ, tumour necrosis factor (TNF)-α and IL-10], produced by these cell populations, in cancer patients (N = 7) undergoing immunotheraphy with autologous DCs.
We observed an initial increase in T helper cells (CD4+) expressing IL-2, IFN-γ, IL-12, TNF-α, and IL-10 after initiation of treatment, with statistically significant for the cytokines IL-2, TNF-α and IL-10. A similar significant effect was observed for IL-2-expressing cytotoxic T cells (CD8+). The percentage of total T cells (CD3+) remained elevated throughout immunotherapy. Regulatory T cells (CD25+/FOXP3+) only showed high percentage of their maximum value when analyzed the pretreatment levels, with statistically significant.
Immunotherapy with DCs stimulated the immune response, as evidenced by an increase in percent fluorescence of most cell populations investigated during the specified treatment period.
癌症源于特定基因突变，导致细胞不受控制地增殖。树突状细胞（DCs）是重要的免疫细胞，在诱导抗肿瘤反应中发挥关键作用。
我们研究了 T 淋巴细胞、辅助性 T 细胞、细胞毒性 T 细胞和调节性 T 细胞介导的免疫反应，以及这些细胞群体产生的细胞因子[白细胞介素（IL）-2、IL-12、干扰素（IFN）-γ、肿瘤坏死因子（TNF）-α 和 IL-10]，这些细胞在接受自体树突状细胞免疫治疗的癌症患者（N=7）中。
我们观察到治疗开始后，表达 IL-2、IFN-γ、IL-12、TNF-α 和 IL-10 的辅助性 T 细胞（CD4+）最初增加，其中细胞因子 IL-2、TNF-α 和 IL-10 具有统计学意义。表达 IL-2 的细胞毒性 T 细胞（CD8+）也观察到类似的显著效应。整个免疫治疗期间，总 T 细胞（CD3+）的百分比保持升高。调节性 T 细胞（CD25+/FOXP3+）仅在分析预处理水平时显示出其最大百分比的高值，具有统计学意义。
DC 免疫疗法刺激了免疫反应，这可通过在指定治疗期间研究的大多数细胞群体的荧光百分比增加来证明。
