Oncology Research Institute (IPON), Federal University of the Triângulo Mineiro (UFTM), Brazil.
Clin Med Insights Oncol. 2013 Jul 28;7:165-72. doi: 10.4137/CMO.S12268. Print 2013.
The objective of this study was to evaluate some of the mechanisms involved in the activation of the immune system in patients with advanced-stage cancer (n = 7) who received an autologous dendritic cell vaccine. We examined the immune response mediated by macrophages (CD14+), natural killer cells (CD56+), and B lymphocytes (CD19+) by flow cytometry and assessed the expression of Th1 (IFN-γ, TNF-α, IL-2, and IL-12), Th2 (IL-4), and Treg (TGF-β) cytokines by flow cytometry and an enzyme-linked immunosorbent assay. The CD14+ TNF-α+ population was significantly increased (P < 0.04) when patients received the vaccine; IL-2 expression in both NK cells and in B lymphocytes was increased after a transient initial increase showed a nearly significant decrease (P < 0.07 and P < 0.06 respectively), whereas the CD19+ and CD56+ populations did not show significant changes. Dendritic cell-based immunotherapy led to increased secretion of IFN-γ and IL-12 and reduced secretion of TGF-β. In conclusion, it is likely that the autologous dendritic cell vaccine stimulated the immune cells from the peripheral blood of patients with cancer and generally increased the production of Th1 cytokines, which are related to immunomodulatory responses against cancer.
本研究的目的是评估晚期癌症患者（n = 7）接受自体树突状细胞疫苗后免疫系统激活所涉及的一些机制。我们通过流式细胞术检查了由巨噬细胞（CD14+）、自然杀伤细胞（CD56+）和 B 淋巴细胞（CD19+）介导的免疫反应，并通过流式细胞术和酶联免疫吸附试验评估了 Th1（IFN-γ、TNF-α、IL-2 和 IL-12）、Th2（IL-4）和 Treg（TGF-β）细胞因子的表达。当患者接受疫苗时，CD14+TNF-α+群体显著增加（P < 0.04）；NK 细胞和 B 淋巴细胞中的 IL-2 表达在短暂的初始增加后呈下降趋势（分别为 P < 0.07 和 P < 0.06），而 CD19+和 CD56+群体没有明显变化。基于树突状细胞的免疫疗法导致 IFN-γ和 IL-12 的分泌增加，而 TGF-β的分泌减少。总之，自体树突状细胞疫苗可能刺激了癌症患者外周血中的免疫细胞，通常增加了与抗肿瘤免疫调节反应相关的 Th1 细胞因子的产生。
