Ou Shiyi, Zhang Jing, Wang Yong, Zhang Ning
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Enzyme Res. 2011;2011:848939. doi: 10.4061/2011/848939. Epub 2011 Mar 31.
A mixture of wheat bran with maize bran as a carbon source and addition of (NH(4))SO(4) as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH(4))SO(4) 1.2 g and MgSO(4) 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.
与仅以麦麸作为碳源和氮源相比,发现以麦麸与玉米麸的混合物作为碳源并添加硫酸铵作为氮源可显著提高阿魏酸酯酶(FAE)的产量。锥形瓶中的最佳条件为碳源(30克)与水比例为1∶1,玉米麸与麦麸比例为1∶2,硫酸铵1.2克,硫酸镁70毫克。在这些条件下,FAE活性为7.68毫单位/克。混合碳源上的FAE活性对培养物中所含的植物细胞壁表现出高活性。