Sakthiselvan Punniavan, Naveena Balakrishnan, Partha Nagarajan
Department of Chemical Engineering A.C. College of Technology Anna University Chennai Chennai India Department of Chemical Engineering, A.C. College of Technology, Anna University Chennai, Chennai, India.
Braz J Microbiol. 2015 Mar 4;45(4):1293-302. doi: 10.1590/s1517-83822014000400020. eCollection 2014.
Xylanase (EC 3. 2. 1. 8), hydrolyzes xylo-oligosaccharides into D-xylose and required for complete hydrolysis of native cellulose and biomass conversion. It has broad range of applications in the pulp and paper, pharmaceutical and Agri-food industries. Fifty fungal species were isolated from the fouled soil around an oil refinery and screened for the production of xylanase enzyme by enrichment culture techniques. The isolated fungal strain was identified as Hypocrea lixii SS1 based on the results of biochemical tests and 18s rRNA sequencing. The phylogenetic tree was constructed using the MEGA 5 software. Further, Hypocrea lixii SS1 was tested for the ability to utilize the sunflower oil sludge (waste from the oil industry) as the sole carbon source for xylanase production. The growth characteristics of Hypocrea lixii SS1 were also studied and maximum growth was found on the 7(th) day of incubation. The fungus showed a remarkable xylanase production of 38.9 U/mL. Xylanase was purified using a combination of 0-50% NH₄SO₂ precipitation, DEAE-sepharose and Sephacryl S-200 chromatography. Single peak obtained in RP-HPLC confirms the purity of xylanase. Further the enzyme produced was affirmed as xylanase with its molecular weight (29 kDa) using SDS-PAGE.
木聚糖酶（EC 3.2.1.8）可将木寡糖水解为D-木糖，是天然纤维素完全水解和生物质转化所必需的。它在制浆造纸、制药和农业食品工业中有广泛应用。从炼油厂周围受污染的土壤中分离出50种真菌，并通过富集培养技术筛选木聚糖酶的产生情况。根据生化试验结果和18s rRNA测序，将分离出的真菌菌株鉴定为绿粘帚霉SS1。使用MEGA 5软件构建系统发育树。此外，还测试了绿粘帚霉SS1利用向日葵油污泥（石油工业废料）作为木聚糖酶生产唯一碳源的能力。还研究了绿粘帚霉SS1的生长特性，发现其在培养第7天生长达到最大值。该真菌产生的木聚糖酶活性显著，为38.9 U/mL。通过0-50%硫酸铵沉淀、DEAE-琼脂糖和Sephacryl S-200色谱相结合的方法纯化木聚糖酶。RP-HPLC中获得的单峰证实了木聚糖酶的纯度。进一步通过SDS-PAGE确定所产生酶为木聚糖酶，其分子量为29 kDa。
