Santuzzi Cíntia Helena, Buss Hygor Franca, Pedrosa Diego França, Freire Martha Oliveira Vieira Moniz, Nogueira Breno Valentim, Gonçalves Washington Luiz Silva
Federal University of Espírito Santo (UFES), Vitória, Espírito Santo, Brazil.
An Bras Dermatol. 2011 Mar-Apr;86(2):278-83. doi: 10.1590/s0365-05962011000200011.
Low level laser therapy and cyclooxygenase-2 (ICOX2) selective inhibitors have been widely used to modulate inflammatory response; however, their effect on wound reepithelialization are not well understood.
To evaluate the isolated and combined effects of low level laser therapy and ICOX2 in the reepithelization of skin incisional wounds in mice.
We induced a 1-cm wound on the back of each mouse, which were divided into four groups (N = 20): control, laser therapy, treated with celecoxib and combined therapy. The animals in the celecoxib and combined therapy groups were treated with celecoxib for 10 days before skin incision. The experimental wounds were irradiated with He-Ne low power laser (632nm, dose: 4J/cm2) in scanning for 12 seconds during three consecutive days in the laser therapy and combined therapy groups. The animals were sacrificed 3 days after surgery. Samples of the wounds were collected and stained (Masson's Trichrome) for histomorphometric analysis.
Both the laser therapy group and the celecoxib group showed an increase in skin reepithelialization compared to the control group; however, the combined therapy group showed no differences. As for keratinization, the laser therapy and combined therapy groups showed a reduction in keratinocytes compared with the control group.
The results show that the use of low level laser therapy and ICOX2 in isolation increases epithelial cells, but only low level laser therapy reduced skin keratinocytes. The combined treatment restores innate epithelialization and decreases keratinization in spite of accelerating wound contraction with improvement in the organization of the wound in the skin of mice.
低强度激光疗法和环氧化酶-2（COX2）选择性抑制剂已被广泛用于调节炎症反应；然而，它们对伤口再上皮化的影响尚不清楚。
评估低强度激光疗法和COX2单独及联合应用对小鼠皮肤切开伤口再上皮化的影响。
在每只小鼠背部诱导一个1厘米的伤口，将其分为四组（每组n = 20）：对照组、激光治疗组、塞来昔布治疗组和联合治疗组。塞来昔布治疗组和联合治疗组的动物在皮肤切开前用塞来昔布治疗10天。激光治疗组和联合治疗组在连续三天内，用氦氖低功率激光（632nm，剂量：4J/cm²）对实验伤口进行扫描照射12秒。术后3天处死动物。收集伤口样本并进行染色（Masson三色染色）以进行组织形态计量分析。
与对照组相比，激光治疗组和塞来昔布组的皮肤再上皮化均增加；然而，联合治疗组无差异。至于角质化，激光治疗组和联合治疗组与对照组相比，角质形成细胞减少。
结果表明，单独使用低强度激光疗法和COX2可增加上皮细胞，但只有低强度激光疗法可减少皮肤角质形成细胞。联合治疗可恢复天然上皮化并减少角质化，尽管在加速伤口收缩的同时改善了小鼠皮肤伤口的组织状态。
