Department of Animal Reproduction Biotechnology and Environmental Hygiene, West Pomeranian University of Technology in Szczecin, Judyma 6 Street, 71-466, Szczecin, Poland.
Biol Trace Elem Res. 2011 Dec;144(1-3):517-24. doi: 10.1007/s12011-011-9075-0. Epub 2011 May 21.
The present study was undertaken to evaluate the concentration of selenium and selected heavy metals and their possible relationship in serum of 25 healthy lactating cows (Montbéliarde) reared in a non-polluted area, in the western part of Poland. Cadmium, lead, copper, and zinc concentrations were determined by inductively coupled plasma-atomic emission spectrometry and Se concentration was determined fluorimetrically. The content of Se, Zn, and Cu was 0.083 ± 0.026, 0.629 ± 0.413, and 0.152 ± 0.042 μg/mL, respectively. The presence of the Cd and Pb was found in all serum samples. The mean concentration of these metals was 0.0009 ± 0.0008 and 0.018 ± 0.016 μg/mL, respectively. Analysis of correlations between Se and toxic metals showed a negative and significant (P < 0.05) relationship between selenium concentration and lead and cadmium concentration in the serum of the animals studied, with correlation coefficients of r = -0.595 and r = -0.618, respectively. For copper and zinc, this relationship was also negative but not significant (r = -0.255 and r = -0.203). Our study demonstrated that the level of toxic metals decreased as serum selenium concentration increased. It seems necessary to conduct further research on the interactions between these elements in blood, serum, and target organs concomitantly with the determination of their intake.
本研究旨在评估 25 头饲养在波兰西部未受污染地区的泌乳荷斯坦奶牛（Montbéliarde）血清中硒和选定重金属的浓度及其可能的关系。采用电感耦合等离子体原子发射光谱法测定镉、铅、铜和锌的浓度，采用荧光法测定硒浓度。硒、锌和铜的含量分别为 0.083 ± 0.026、0.629 ± 0.413 和 0.152 ± 0.042 μg/mL。所有血清样本中均发现存在 Cd 和 Pb。这些金属的平均浓度分别为 0.0009 ± 0.0008 和 0.018 ± 0.016 μg/mL。对硒与有毒金属之间相关性的分析表明，在所研究动物的血清中，硒浓度与铅和镉浓度之间存在负相关且有统计学意义（P < 0.05），相关系数分别为 r = -0.595 和 r = -0.618。对于铜和锌，这种关系也是负相关的，但没有统计学意义（r = -0.255 和 r = -0.203）。本研究表明，随着血清硒浓度的升高，有毒金属的水平降低。似乎有必要在同时测定这些元素的摄入量的情况下，对血液、血清和靶器官中这些元素之间的相互作用进行进一步研究。
