Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Biol Trace Elem Res. 2010 Jan;133(1):1-11. doi: 10.1007/s12011-009-8405-y. Epub 2009 May 26.
A total of 73 blood samples (56 from smokers and 17 from nonsmokers) were collected to determine the concentrations of selected heavy metal in the whole blood of smokers and nonsmokers living in and around the city of Amman, Jordan. Analysis of heavy metals in the whole blood samples of various groups took in consideration the number of cigarettes smoked per day. The analysis of blood samples was carried out using inductively coupled plasma optical emission spectrometry. This study aimed to evaluate the blood metal levels in smokers and nonsmokers and to assess the influence of smoking cigarettes on blood metal levels. The results were compared with those from a control group. The results indicated that the average concentrations of cadmium (Cd), lead (Pb), copper (Cu), zinc (Zn), and selenium (Se) were 0.0313, 0.344, 2.328, 3.214, and 0.332 mg/L, respectively. Statistical analysis of results indicated that these average concentrations were significantly higher compared with the average concentrations in nonsmokers (P < 0.05). Moreover, the correlations between blood metal and other blood metal levels in smokers, the correlations between blood metal and other blood metal levels in nonsmokers, and the correlations between blood metal concentration in smokers and its concentration in nonsmokers were calculated. The standard reference material (blood serum National Institute of Standards and Technology 1598) and the quality control were used to validate the reliability of the method used for the estimation of heavy metals in blood samples. Results revealed that there was an agreement between the certified values and the measured values.
共采集了 73 份血样（56 份来自吸烟者，17 份来自不吸烟者），以确定居住在约旦安曼市及其周边地区的吸烟者和不吸烟者全血中选定重金属的浓度。在考虑每天吸烟数量的情况下，对不同人群的全血样本中的重金属进行了分析。采用电感耦合等离子体发射光谱法对血样进行了分析。本研究旨在评估吸烟者和不吸烟者的血液金属水平，并评估吸烟对血液金属水平的影响。结果与对照组进行了比较。结果表明，镉（Cd）、铅（Pb）、铜（Cu）、锌（Zn）和硒（Se）的平均浓度分别为 0.0313、0.344、2.328、3.214 和 0.332mg/L。结果的统计分析表明，这些平均浓度明显高于不吸烟者的平均浓度（P<0.05）。此外，还计算了吸烟者血液金属与其他血液金属水平之间的相关性、不吸烟者血液金属与其他血液金属水平之间的相关性，以及吸烟者血液金属浓度与其在不吸烟者血液中的浓度之间的相关性。使用标准参考物质（血清国家标准与技术研究所 1598）和质量控制来验证用于估算血液样本中重金属的方法的可靠性。结果表明，测定值与证书值之间存在一致性。
