Department of Surgery, Akita University School of Medicine, Akita City, Japan.
Ann Surg Oncol. 2011 Nov;18(12):3377-83. doi: 10.1245/s10434-011-1798-9. Epub 2011 May 21.
The cation channels of the transient receptor potential (TRP) superfamily are implicated in cancer formation; in particular, TRPC3 has been shown to contribute to the progression of human ovarian cancer. However, the relationship between TRP expression and the clinicopathological characteristics of lung cancer and patient prognosis is not well understood. Therefore, we investigated the relationship between TRP expression and the prognosis of patients with adenocarcinoma of the lung.
We used semiquantitative real-time reverse transcription polymerase chain reaction to assess the expression of TRP mRNA in tumor samples from 95 patients with adenocarcinoma of the lung. We then correlated the TRP mRNA levels with clinicopathological factors. We also used immunohistochemical staining to determine the localization of expressed TRP.
The 5-year overall and disease-free survival rates among patients expressing higher levels of TRPC3 mRNA were significantly better than the corresponding rates among patients expressing lower levels (P=0.004, P=0.002, respectively, by log-rank test). Multivariate Cox proportional hazard analyses revealed that tumor size (hazard ratio, 2.46; 95% confidence interval [CI], 1.06 to 5.79; P=0.036), n2 (hazard ratio, 3.81; 95% CI, 1.29 to 11.77; P=0.015) and TRPC3 (hazard ratio, 2.71; 95% CI, 1.33 to 5.59; P=0.006) were independent factors affecting the 5-year overall survival rate. Immunohistochemistry showed that the cytoplasm of tumor cells were stained positively for TRPC3.
Higher levels of TRPC3 expression in tumor cells are an independent predictor of a better prognosis in patients with adenocarcinoma of the lung.
瞬时受体电位 (TRP) 超家族的阳离子通道与癌症的形成有关；特别是 TRPC3 已被证明有助于人类卵巢癌的进展。然而，TRP 表达与肺癌的临床病理特征和患者预后之间的关系尚不清楚。因此，我们研究了 TRP 表达与肺腺癌患者预后的关系。
我们使用半定量实时逆转录聚合酶链反应评估了 95 例肺腺癌患者肿瘤样本中 TRP mRNA 的表达。然后，我们将 TRP mRNA 水平与临床病理因素相关联。我们还使用免疫组织化学染色来确定表达的 TRP 的定位。
表达更高水平的 TRPC3 mRNA 的患者的 5 年总生存率和无病生存率明显好于表达较低水平的患者（对数秩检验，P=0.004，P=0.002）。多变量 Cox 比例风险分析显示肿瘤大小（危险比，2.46；95%置信区间 [CI]，1.06 至 5.79；P=0.036）、n2（危险比，3.81；95%CI，1.29 至 11.77；P=0.015）和 TRPC3（危险比，2.71；95%CI，1.33 至 5.59；P=0.006）是影响 5 年总生存率的独立因素。免疫组织化学显示肿瘤细胞的细胞质对 TRPC3 呈阳性染色。
肿瘤细胞中 TRPC3 表达水平较高是肺腺癌患者预后较好的独立预测因素。
