Ishida Yuko, Ozaki Mamiko
Department of Biology, Graduate School of Science, Kobe University, Rokkodai-cho, Nada-ku, Kobe, Japan,
Naturwissenschaften. 2011 Jul;98(7):635-8. doi: 10.1007/s00114-011-0806-z. Epub 2011 May 21.
In the blowfly Phormia regina, experience of simultaneous feeding with D: -limonene exposure inhibits proboscis extension reflex (PER) due to decreased tyramine (TA) titer in the brain. To elucidate the molecular mechanism of TA signaling pathway related to the associated feeding behavior, we cloned cDNA encoding the octopamine/TA receptor (PregOAR/TAR). The deduced protein is composed of 607 amino acid residues and has 7 predicted transmembrane domains. Based on homology and phylogenetic analyses, this protein belongs to the OAR/TAR family. The PregOAR/TAR was mainly expressed in head, with low levels of expression in other tissues at adult stages. Gene expression profile is in agreement with a plethora of functions ascribed to TA in various insect tissues. The immunolabeled cell bodies and processes were localized in the medial protocerebrum, outer layer of lobula, antennal lobe, and subesophageal ganglion. These results suggest that decrease of TA level in the brain likely affects neurons expressing PregOAR/TAR, causing mediation of the sensitivity in the sensillum and/or output of motor neurons for PER.
在丽蝇（Phormia regina）中，同时经历取食和接触D-柠檬烯会抑制伸吻反射（PER），这是由于脑中酪胺（TA）滴度降低所致。为阐明与相关取食行为有关的TA信号通路的分子机制，我们克隆了编码章鱼胺/TA受体（PregOAR/TAR）的cDNA。推导的蛋白质由607个氨基酸残基组成，有7个预测的跨膜结构域。基于同源性和系统发育分析，该蛋白质属于OAR/TAR家族。PregOAR/TAR主要在头部表达，在成虫阶段其他组织中的表达水平较低。基因表达谱与TA在各种昆虫组织中具有的多种功能一致。免疫标记的细胞体和突起定位于中脑、小叶外层、触角叶和咽下神经节。这些结果表明，脑中TA水平的降低可能会影响表达PregOAR/TAR的神经元，导致感觉毛敏感性的调节和/或伸吻反射运动神经元的输出。
