Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Arch Virol. 2011 Sep;156(9):1671-5. doi: 10.1007/s00705-011-1022-z. Epub 2011 May 22.
In this study, we first investigated the prevalence of astrovirus in stools of dogs with and without diarrhea in Shanghai, China. Of all the specimens, 22 (12.02%) from the 183 dogs with diarrhea and none (0%) from the 138 healthy controls were positive for astrovirus. Furthermore, we cloned partial sequences of ORF1b (442 bp) and the entire sequences of ORF2 (2475 bp). Phylogenetic analysis showed that the new isolates were belonged to genus Mamastrovirus and most closely clustered with the Italy strain, based on the ORF2 sequences available. However, the new isolates and the Italy strain were divided into two different clusters. The new isolates may be a new strain of canine astrovirus.
在这项研究中，我们首先调查了在中国上海，腹泻和非腹泻犬粪便中星状病毒的流行情况。在所有标本中，183 只腹泻犬中有 22 只（12.02%）和 138 只健康对照犬中没有（0%）星状病毒阳性。此外，我们克隆了 ORF1b（442 bp）的部分序列和 ORF2（2475 bp）的全长序列。基于 ORF2 序列的系统发育分析表明，新分离株属于 Mamastrovirus 属，与意大利株最为密切聚类。然而，新分离株和意大利株分为两个不同的簇。新分离株可能是犬星状病毒的一个新株。
