Wang Wenjie, Zhang Ya, Wang Xiang, Zhu Xu, Gong Li, Jing Zheng, Meng Ting, Shen Jiayu, Tan Yeping, Qin Tong, Li Zhimin, Bi Zhenwei
School of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, China.
Front Vet Sci. 2025 Jul 22;12:1614686. doi: 10.3389/fvets.2025.1614686. eCollection 2025.
Feline astroviruses (FeAstVs) have been increasingly detected in cats in recent years, yet their isolation and pathogenicity remain poorly characterized. In this study, we screened 86 feline diarrheal samples and identified FeAstV in 9.3% (8/86) of the cases, most of which were co-infected with feline parvovirus (FPV). A FeAstV strain (22SDWH1003-16) was successfully isolated in F81 cells from a single FeAstV positive sample, inducing cytopathic effects (CPEs) over 15 passages. The phylogenetic tree of ORF2 classified the isolate within group 1, the most common in the inter-specific transmission within cats. Experimental inoculation of four cats revealed seroconversion in all animals, transient fecal shedding in 3/4 cats, and self-limiting diarrhea in one individual. Co-infection experiments demonstrated enhanced FeAstV replication in the presence of FPV. Our findings provide the direct evidence of FeAstV-induced diarrhea in cats and highlight the role of viral co-infections in disease severity.
近年来，猫星状病毒（FeAstVs）在猫中的检出率越来越高，但其分离和致病性仍未得到充分表征。在本研究中，我们筛查了86份猫腹泻样本，在9.3%（8/86）的病例中鉴定出FeAstV，其中大多数与猫细小病毒（FPV）共同感染。从一份FeAstV阳性样本中成功在F81细胞中分离出一株FeAstV毒株（22SDWH1003-16），在15代培养中诱导出细胞病变效应（CPEs）。ORF2的系统发育树将该分离株归类为第1组，这是猫种间传播中最常见的组。对四只猫进行实验接种后发现，所有动物均出现血清转化，3/4的猫出现短暂粪便排毒，一只猫出现自限性腹泻。共感染实验表明，在存在FPV的情况下，FeAstV的复制增强。我们的研究结果提供了FeAstV引起猫腹泻的直接证据，并突出了病毒共感染在疾病严重程度中的作用。
