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逆转录病毒载体将基因转移到原代培养的正常和成肌细胞突变体中，这些基因在肌管中得以表达。

Genes transferred by retroviral vectors into normal and mutant myoblasts in primary cultures are expressed in myotubes.

作者信息

Smith B F, Hoffman R K, Giger U, Wolfe J H

机构信息

School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.

出版信息

Mol Cell Biol. 1990 Jun;10(6):3268-71. doi: 10.1128/mcb.10.6.3268-3271.1990.

DOI:10.1128/mcb.10.6.3268-3271.1990
PMID:2160598
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360696/
Abstract

Retroviral vectors were used to transfer genes efficiently into rat and dog myoblasts in primary cultures under conditions which permitted the transduced myoblasts to differentiate into myotubes expressing the transferred genes. The transduced myotubes expressed normal markers of differentiation and were morphologically indistinguishable from uninfected myotubes. Retroviral vector-mediated gene transfer was also used to correct a genetic enzyme deficiency in mutant canine muscle cells.

摘要

在允许转导的成肌细胞分化为表达所转移基因的肌管的条件下，逆转录病毒载体被用于将基因高效地转移到原代培养的大鼠和犬成肌细胞中。转导的肌管表达正常的分化标志物，并且在形态上与未感染的肌管无法区分。逆转录病毒载体介导的基因转移也被用于纠正突变犬肌肉细胞中的遗传酶缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5d6/360696/f5546fe352df/molcellb00042-0840-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5d6/360696/31828b73552a/molcellb00042-0839-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5d6/360696/f5546fe352df/molcellb00042-0840-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5d6/360696/31828b73552a/molcellb00042-0839-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5d6/360696/f5546fe352df/molcellb00042-0840-a.jpg

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本文引用的文献

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Beta-glucuronidase deficiency in a dog: a model of human mucopolysaccharidosis VII.犬β-葡萄糖醛酸酶缺乏症：人类黏多糖贮积症VII型的一种模型。
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Muscle cell culture as a tool in animal growth research.肌肉细胞培养作为动物生长研究中的一种工具。
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Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production.逆转录病毒包装细胞系的重新设计，以避免重组导致辅助病毒产生。
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