理性设计南极假丝酵母脂肪酶 B 用于 D,D-丙交酯的开环聚合。
Rational redesign of Candida antarctica lipase B for the ring opening polymerization of D,D-lactide.
机构信息
Royal Institute of Technology, School of Biotechnology, Department of Biochemistry, AlbaNova University Centre, 106 91 Stockholm, Sweden.
出版信息
Chem Commun (Camb). 2011 Jul 14;47(26):7392-4. doi: 10.1039/c1cc10865d. Epub 2011 May 23.
PMID:21607247
Abstract
Based on molecular modelling, the enzyme Candida antarctica lipase B was redesigned as a catalyst for the ring opening polymerization of D,D-lactide. Two mutants with 90-fold increased activity as compared to the wild-type enzyme were created. In a preparative synthesis of poly(D,D-lactide) the mutants greatly improved the rate and the degree of polymerization.
摘要
基于分子建模，对南极假丝酵母脂肪酶 B 进行了重新设计，作为 D,D-丙交酯开环聚合的催化剂。与野生型酶相比，两种突变体的活性提高了 90 倍。在聚（D,D-丙交酯）的制备合成中，突变体大大提高了聚合速率和聚合度。
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