Zusman I, Zusman R, Korol D
Oncol Rep. 1994 Jul;1(4):751-4. doi: 10.3892/or.1.4.751.
A new, effective support for the isolation of proteins has been invented utilizing a gel fiberglass (GFG) (R. Zusman, Patent application 1992, 1993). We describe the utilization of this support to isolate tumor-associated proteins (TAP) from sera of colon tumor-bearing rats. Sera were percolated through GFG columns with entrapped anti-rat colon cancer IgG from rabbits. TAP were eluted in a large amount, up to 3 mg/ml sera/column. Their affinity to antitumorous polyclonal antibodies was detected by ELISA. Western immunoblotting with commercial monoclonal antibodies has identified the isolated antigens as p21 and p53. Their concentrations were much higher (up to 1,000 times) in sera from tumor-bearing rats compared to control rats. The method was highly reproducible. It is suggested that data obtained can be considered as a basis for the further improving of quantitative methods of diagnosis.
利用凝胶玻璃纤维（GFG）发明了一种用于蛋白质分离的新型有效载体（R. 祖斯曼，专利申请，1992年，1993年）。我们描述了利用这种载体从荷瘤大鼠血清中分离肿瘤相关蛋白（TAP）的方法。血清通过装有兔抗大鼠结肠癌IgG的GFG柱进行渗滤。大量TAP被洗脱出来，每毫升血清/柱可达3毫克。通过ELISA检测它们与抗肿瘤多克隆抗体的亲和力。用商业单克隆抗体进行的Western免疫印迹法已将分离出的抗原鉴定为p21和p53。与对照大鼠相比，荷瘤大鼠血清中它们的浓度要高得多（高达1000倍）。该方法具有高度可重复性。建议所获得的数据可作为进一步改进定量诊断方法的基础。
