Sandler B, Smirnoff P, Tendelr Y, Zinder O, Zusman R, Zusman I
Laboratory of Teratology and Experimental Oncology, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Int J Mol Med. 1998 Apr;1(4):767-70. doi: 10.3892/ijmm.1.4.767.
The possibility to use anti-p53 IgG for isolation of the soluble p53 antigen as a serological tumor marker has been shown in our previous studies. In order to prove the specificity of such IgG, we compared the effectiveness of columns with anti-p53 IgG and IgG isolated from non-treated rabbits (regular IgG). The gel fiberglass (GFG) columns for affinity chromatography were prepared separately with both types of IgG. The same serum was percolated simultaneously through both columns and the results of elution were compared. The total concentration of tumor-associated antigens (TAA) eluted from the serum of cancer patients was similar in both TAA mixtures isolated either with anti-p53 IgG or with the regular IgG. Differences are manifest in the content of total proteins and amount of each eluted protein: a mixture eluted with the regular IgG contains several proteins whereas the anti-p53 IgG isolated only two proteins, p64 and p53. The amount of the soluble p53 antigen isolated from the cancer serum was significantly higher when it was isolated with anti-p53 IgG. The method developed in our laboratory was shown to be highly specific both to isolate proteins related to cancer (p53) and non-cancer disorders (p64). Data presented in this report show that such specificity can be achieved only if the anti-p53 IgG is used: the regular IgG obtained from non-treated animals isolate many proteins among which the concentration of specific p53 protein is lost.
在我们之前的研究中，已表明使用抗 p53 IgG 分离可溶性 p53 抗原作为血清学肿瘤标志物的可能性。为了证明此类 IgG 的特异性，我们比较了装有抗 p53 IgG 的柱和从未经处理的兔子分离得到的 IgG（常规 IgG）的柱的效果。分别用这两种类型的 IgG 制备用于亲和层析的凝胶玻璃纤维（GFG）柱。将同一份血清同时通过两根柱，并比较洗脱结果。从癌症患者血清中洗脱的肿瘤相关抗原（TAA）的总浓度在使用抗 p53 IgG 或常规 IgG 分离得到的两种 TAA 混合物中相似。差异体现在总蛋白含量和每种洗脱蛋白的量上：用常规 IgG 洗脱的混合物含有几种蛋白，而抗 p53 IgG 仅分离出两种蛋白，即 p64 和 p53。当用抗 p53 IgG 从癌症血清中分离可溶性 p53 抗原时，其含量显著更高。我们实验室开发的方法在分离与癌症相关的蛋白（p53）和非癌症疾病相关的蛋白（p64）方面均显示出高度特异性。本报告中的数据表明，只有使用抗 p53 IgG 才能实现这种特异性：从未经处理的动物获得的常规 IgG 分离出许多蛋白，其中特异性 p53 蛋白的浓度丢失了。
