Sandler B, Smirnoff P, Shani A, Idelevich E, Pfefferman R, Davidovich B, Zusman R, Zusman I
Laboratory of Teratology and Experimental Oncology, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Int J Mol Med. 1998 Feb;1(2):453-7. doi: 10.3892/ijmm.1.2.453.
The role of various serological tumor markers, p53 soluble antigen and its autoantibodies, was evaluated for the detection of colon cancer in humans. An HPLC technique was used to measure serum levels of both forms of p53 protein after their partial isolation on gel fiberglass affinity chromatography columns. Tumor-associated proteins (TAP) in the form of either antigens or autoantibodies were about 4% of the total protein isolated from the serum of colon cancer patients. The absolute amount of each of the two types of TAP was also similar: 14.69 2.88 and 18.29 3.85 mg ml-1, respectively. The amount of p53 autoantibodies was higher than those of p53 antigen, but the difference was not significant: 9.35 3.48 and 6. 19 3.87 mg/ml, respectively (p>0.05). A good correlation was found between the total amount of tumor-associated antigens (TAA) and the amount of p53 antigen (r=0.69), total amount of IgG and the amount of p53 autoantibodies (r=0.46), and between both forms of p53 protein (r=0.46). A high coefficient of regression was found between the total amount of TAA and the amount of p53 antigen (b=2.4). Relationships between Duke's stage in colon cancer and the serum levels of p53 protein were weak: 0.33 and -0.32 for p53 antigen and its autoantibodies, respectively. Serum determination of p53 autoantibodies has no advantage over the determination of p53 antigen. Both forms of p53 protein can be isolated with extremely high accuracy for the pathological diagnosis of cancer (87-93%). Specificity of the method was proved using of commercial p53 PAb OD1: the GFG columns with this antibody were able to isolate the same proteins which were isolated by GFG columns with anti-p53 IgG. Moreover, the isolation of p53 protein was performed with higher effectiveness using the GFG columns with entrapped anti-p53 IgG than those columns with commercial DO1 PAb.
评估了各种血清学肿瘤标志物、p53可溶性抗原及其自身抗体在人类结肠癌检测中的作用。在凝胶玻璃纤维亲和色谱柱上对p53蛋白的两种形式进行部分分离后，采用高效液相色谱技术测量血清中这两种形式p53蛋白的水平。以抗原或自身抗体形式存在的肿瘤相关蛋白（TAP）约占从结肠癌患者血清中分离出的总蛋白的4%。两种类型的TAP各自的绝对量也相似：分别为14.69±2.88和18.29±3.85mg/ml。p53自身抗体的量高于p53抗原，但差异不显著：分别为9.35±3.48和6.19±3.87mg/ml（p>0.05）。发现肿瘤相关抗原（TAA）总量与p53抗原量之间存在良好相关性（r=0.69），IgG总量与p53自身抗体量之间存在良好相关性（r=0.46），并且p53蛋白的两种形式之间也存在良好相关性（r=0.46）。发现TAA总量与p53抗原量之间的回归系数较高（b=2.4）。结肠癌的杜克分期与p53蛋白血清水平之间的关系较弱：p53抗原及其自身抗体的相关系数分别为0.33和-0.32。血清中p53自身抗体的测定相对于p53抗原的测定没有优势。两种形式的p�3蛋白都能以极高的准确性分离出来用于癌症的病理诊断（87-93%）。使用商业p53 PAb OD1证明了该方法的特异性：带有这种抗体的GFG柱能够分离出与带有抗p53 IgG的GFG柱所分离的相同蛋白质。此外，使用包埋有抗p53 IgG的GFG柱比使用带有商业DO1 PAb的柱更有效地分离p53蛋白。
