Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Dev Dyn. 2011 Jul;240(7):1639-49. doi: 10.1002/dvdy.22666. Epub 2011 May 23.
To understand the developmental mechanism that determines limb size and the consequent limb-to-trunk proportions in the tetrapod body, we investigated the role of the paraxial mesoderm in the specification of the limb and flank fields in the chick embryo. We found that the paraxial mesoderm subjacent to the limb field can affect the size of the limb bud along the anterior-posterior and proximal-distal axes. We also found that the paraxial mesoderm subjacent to the flank plays roles in suppressing the emergence and growth of the limb bud and in promoting flank-specific apoptosis in the lateral plate mesoderm. Our results suggest that signals from the paraxial mesoderm specify the limb and flank fields in the competent lateral plate mesoderm.
为了理解决定四肢大小和四足动物身体四肢与躯干比例的发育机制，我们研究了在鸡胚中轴中胚层在肢体和侧腹区域的特化中的作用。我们发现，位于肢体区域下方的中胚层可以影响肢体芽沿前后和远近轴的大小。我们还发现，位于侧腹下方的中胚层在抑制肢体芽的出现和生长以及促进侧方板中胚层中侧翼特异性细胞凋亡方面发挥作用。我们的结果表明，从中胚层发出的信号在有能力的侧方板中胚层中指定了肢体和侧腹区域。
