Klein R, Conway D, Parada L F, Barbacid M
Department of Molecular Biology, Squibb Institute for Medical Research, Princeton, New Jersey 08543-4000.
Cell. 1990 May 18;61(4):647-56. doi: 10.1016/0092-8674(90)90476-u.
We previously identified two tyrosine protein kinase genes, designated trk and trkB, that code for putative neurogenic cell surface receptors. In this study, we report that the mouse trkB locus codes for at least two classes of receptor-like molecules. These trkB proteins, designated gp145trkB and gp95trkB, have identical extracellular and transmembrane domains, suggesting that they might recognize the same ligand(s). However, only gp145trkB contains a long cytoplasmic region, which includes a catalytic tyrosine protein kinase domain. trkB transcripts coding for this protein were observed in the cerebral cortex and the pyramidal cell layer of the hippocampus. In contrast, transcripts coding for the noncatalytic gp95trkB molecule were found in the ependymal linings of the cerebral ventricles and in the choroid plexus. These results illustrate that a tyrosine protein kinase locus can code for two structurally and functionally distinct cellular receptors.
我们之前鉴定出了两个酪氨酸蛋白激酶基因，分别命名为trk和trkB，它们编码假定的神经源性细胞表面受体。在本研究中，我们报告小鼠trkB基因座编码至少两类受体样分子。这些trkB蛋白，命名为gp145trkB和gp95trkB，具有相同的细胞外和跨膜结构域，这表明它们可能识别相同的配体。然而，只有gp145trkB包含一个长的细胞质区域，其中包括一个催化性酪氨酸蛋白激酶结构域。在大脑皮层和海马体的锥体细胞层中观察到编码该蛋白的trkB转录本。相比之下，在脑室的室管膜内衬和脉络丛中发现了编码无催化活性的gp95trkB分子的转录本。这些结果表明，一个酪氨酸蛋白激酶基因座可以编码两种结构和功能不同的细胞受体。
