Kapadia C R, Cutruzzola F W, O'Brien K M, Stetz M L, Enriquez R, Deckelbaum L I
Department of Medicine, West Haven Veterans Administration Medical Center, Connecticut.
Gastroenterology. 1990 Jul;99(1):150-7. doi: 10.1016/0016-5085(90)91242-x.
To evaluate the potential of laser-induced fluorescence spectroscopy for the detection of premalignant lesions of the gastrointestinal tract, the hypothesis that adenomatous transformation of colonic mucosa results in an alteration of laser-induced fluorescence that enables its differentiation from normal or hyperplastic tissue was tested. A fiberoptic catheter coupled to a helium-cadmium laser (325 nm) and an optical multichannel analyzer were used to obtain fluorescence spectra (350-600 nm) from 35 normal colonic specimens and 35 resected adenomatous polyps. A score based on six wavelengths was derived by stepwise multivariate linear regression analysis of the spectra. The mean score (+/- SEM) was + 0.86 +/- 0.06 for normal mucosa and -0.86 +/- 0.06 for adenomatous polyps (P less than 0.001). Spectra from an additional 34 normal specimens, 16 adenomatous polyps, and 16 hyperplastic polyps were prospectively classified with accuracies of 100%, 100%, and 94%, respectively. The mean score for hyperplastic polyps was significantly different from adenomatous (P less than 0.001) but not from normal tissue. Thus, quantitative analysis of fluorescence spectra enables the detection of adenomatous transformation in colonic mucosa.
为评估激光诱导荧光光谱技术检测胃肠道癌前病变的潜力，对结肠黏膜腺瘤样转变会导致激光诱导荧光改变从而使其能与正常或增生组织区分开来这一假说进行了验证。使用一根与氦镉激光器（325纳米）相连的光纤导管及一台光学多道分析仪，从35个正常结肠标本和35个切除的腺瘤样息肉中获取荧光光谱（350 - 600纳米）。通过对光谱进行逐步多元线性回归分析得出基于六个波长的分数。正常黏膜的平均分数（±标准误）为 +0.86 ± 0.06，腺瘤样息肉的平均分数为 -0.86 ± 0.06（P < 0.001）。另外34个正常标本、16个腺瘤样息肉和16个增生性息肉的光谱经前瞻性分类，准确率分别为100%、100%和94%。增生性息肉的平均分数与腺瘤样息肉有显著差异（P < 0.001），但与正常组织无显著差异。因此，荧光光谱的定量分析能够检测结肠黏膜中的腺瘤样转变。
