Fiarman G S, Nathanson M H, West A B, Deckelbaum L I, Kelly L, Kapadia C R
Department of Medicine, West Haven Veterans Administration Medical Center, Connecticut 06516, USA.
Dig Dis Sci. 1995 Jun;40(6):1261-8. doi: 10.1007/BF02065535.
Laser-induced autofluorescence has been used to discriminate normal from adenomatous colonic mucosa. However, few studies to date have studied the origin of colonic autofluorescence. Using confocal microscopy (excitation wavelength 488 nm), we have shown that autofluorescence at this wavelength is present predominantly in the lamina propria of normal mucosa but in the epithelium in adenomatous and hyperplastic polyps. The intensity ratio of epithelial cell to lamina propria fluorescence was significantly lower (P < 0.0001) in normal mucosa (0.52 +/- 0.01) compared with either adenomatous (1.6 +/- 0.2) or hyperplastic polyps (1.7 +/- 0.15). However, the ratios were not significantly different between hyperplastic and adenomatous polyps. Thus, confocal microscopy enables the detection of the sites of autofluorescence within colonic mucosa and the quantitation of differences in fluorescence between different tissue types.
激光诱导自体荧光已被用于区分正常结肠黏膜和腺瘤性结肠黏膜。然而，迄今为止，很少有研究探讨结肠自体荧光的来源。使用共聚焦显微镜（激发波长488nm），我们发现该波长下的自体荧光主要存在于正常黏膜的固有层，但在腺瘤性息肉和增生性息肉的上皮中也有。与腺瘤性息肉（1.6±0.2）或增生性息肉（1.7±0.15）相比，正常黏膜中上皮细胞与固有层荧光的强度比显著更低（P<0.0001）（0.52±0.01）。然而，增生性息肉和腺瘤性息肉之间的该比值没有显著差异。因此，共聚焦显微镜能够检测结肠黏膜内自体荧光的部位，并对不同组织类型之间的荧光差异进行定量分析。
