Schomacker K T, Frisoli J K, Compton C C, Flotte T J, Richter J M, Deutsch T F, Nishioka N S
Gastrointestinal Unit, Massachusetts General Hospital, Boston.
Gastroenterology. 1992 Apr;102(4 Pt 1):1155-60.
Ultraviolet laser-induced fluorescence was examined in vivo to determine whether the technique can reliably distinguish between hyperplastic and adenomatous polyps of the colon. Spectra from 86 normal colonic sites, 35 hyperplastic polyps, and 49 adenomatous polyps were recorded in vivo. Polyp type was independently determined by two senior pathologists who were unaware of the fluorescence measurement. A multivariate linear regression analysis was used to differentiate spectra from hyperplastic and adenomatous polyps and resulted in a sensitivity, specificity, predictive value positive, and predictive value negative for identifying adenomatous polyps of 86%, 80%, 86%, and 80%, respectively. These values were not significantly different from the accuracy of routine clinical pathology. Thus, ultraviolet laser-induced fluorescence appears to show promise as a means for distinguishing tissue types. However, further experience is needed before its routine clinical use can be recommended. Significant changes in the fluorescence spectra occurred postmortem, suggesting that future studies of laser-induced fluorescence of colonic tissue must use data acquired in vivo.
对紫外线激光诱导荧光进行了体内检测，以确定该技术能否可靠地区分结肠增生性息肉和腺瘤性息肉。记录了来自86个正常结肠部位、35个增生性息肉和49个腺瘤性息肉的体内光谱。息肉类型由两名不了解荧光测量情况的资深病理学家独立确定。采用多元线性回归分析来区分增生性息肉和腺瘤性息肉的光谱，结果显示识别腺瘤性息肉的灵敏度、特异度、阳性预测值和阴性预测值分别为86%、80%、86%和80%。这些值与常规临床病理学的准确性无显著差异。因此，紫外线激光诱导荧光似乎有望成为区分组织类型的一种手段。然而，在推荐其常规临床应用之前，还需要更多经验。死后荧光光谱发生了显著变化，这表明未来对结肠组织激光诱导荧光的研究必须使用体内获取的数据。
