Coda Sergio, Thompson Alex J, Kennedy Gordon T, Roche Kim L, Ayaru Lakshmana, Bansi Devinder S, Stamp Gordon W, Thillainayagam Andrew V, French Paul M W, Dunsby Chris
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK ; These authors contributed equally to this work.
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; These authors contributed equally to this work.
Biomed Opt Express. 2014 Jan 16;5(2):515-38. doi: 10.1364/BOE.5.000515. eCollection 2014 Feb 1.
We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime -570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.
我们展示了一项对总共47份来自结肠的内镜切除活检/切除标本进行时间和光谱分辨自体荧光的离体研究，使用波长为375nm和435nm的脉冲激发激光源。对来自同一患者的正常和肿瘤性（腺瘤性息肉）组织标本进行配对分析，结果显示平均光谱平均自体荧光寿命存在显著差异——570±740皮秒（p = 0.021，n = 12）。我们还在少数标本中研究了非肿瘤性息肉（n = 6）和炎症性肠病（n = 4）与正常组织相比的荧光特征。
