Materials Science and Engineering Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.
Nanotoxicology. 2011 Jun;5(2):140-56. doi: 10.3109/17435390.2010.504311. Epub 2010 Aug 23.
The dispersion stabilization of silver nanoparticles (AgNPs) in synthetic lung fluid was studied to interrogate the effects on colloidal stability due to the principal constituents of the fluid. The colloidal stability of 20 nm citrate-AgNPs dispersed in the presence of each constituent of the synthetic lung fluid (individually, the complete fluid, and without additives) was observed during titration of increasing sodium chloride concentration. A variety of complementary in situ measurement techniques were utilized, including dynamic light scattering, ultraviolet-visible absorption spectroscopy, atomic force microscopy, and small-angle X-ray scattering, which provided a collective set of information that enabled far better understanding of the dispersion behavior in the fluid than any one technique alone. It was observed that AgNPs continued to adsorb bovine serum albumin (BSA) protein from the synthetic lung fluid solution as the sodium chloride concentration increased, until a maximum BSA coating was achieved prior to reaching the physiological sodium chloride concentration of 154 mmol L(-1). BSA was determined to be the constituent of the synthetic lung fluid that is required to provide colloidal stability at high salt loadings, though the phospholipid constituent exerts a subtle effect. Additionally, as AgNPs are a distinctly different class of nanoparticles apart from the carbon nanotubes and titanium dioxide nanoparticles initially reported to be dispersible using this fluid, this work also demonstrates the broad applicability of synthetic lung fluid in providing stable dispersions for engineered nanoparticles for use in biological assays.
研究了合成肺液中银纳米粒子（AgNPs）的分散稳定性，以探究由于该液体的主要成分对胶体稳定性的影响。在不断增加氯化钠浓度的滴定过程中，观察到分散在合成肺液各成分（单独、完整液体和无添加剂）存在下的 20nm 柠檬酸钠-AgNPs 的胶体稳定性。采用了多种互补的原位测量技术，包括动态光散射、紫外-可见吸收光谱、原子力显微镜和小角 X 射线散射，这些技术提供了一套综合信息，使我们能够更好地理解胶体在该液体中的分散行为，而不是仅依靠任何一种技术。结果表明，随着氯化钠浓度的增加，AgNPs 继续从合成肺液溶液中吸附牛血清白蛋白（BSA）蛋白，直到在达到生理氯化钠浓度 154mmol L(-1)之前达到最大 BSA 涂层。BSA 被确定为在高盐负荷下提供胶体稳定性所需的合成肺液成分，尽管磷脂成分有细微的影响。此外，由于 AgNPs 是一种明显不同于最初报道的可在该液体中分散的碳纳米管和二氧化钛纳米粒子的纳米粒子，因此这项工作还证明了合成肺液在为用于生物测定的工程纳米粒子提供稳定分散方面的广泛适用性。
