Kolomin Timur, Shadrina Maria, Andreeva Lyudmila, Slominsky Petr, Limborska Svetlana, Myasoedov Nikolay
The Laboratory of Molecular Genetics of Hereditary Diseases, Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia.
Regul Pept. 2011 Oct 10;170(1-3):18-23. doi: 10.1016/j.regpep.2011.05.001. Epub 2011 May 24.
Previous studies have shown that synthetic tuftsin analogue Selank causes a transcriptomic response in the rat hippocampus and in spleen cells and may participate in the regulation of inflammatory processes in the body. In this work we studied the effect of Selank and two of its fragments on the expression of genes involved in processes of inflammation. We analyzed the expression of 84 genes involved in processes of inflammation (e.g., chemokines, cytokines, and its receptors) in mouse spleen 6 and 24 h after Selank single intraperitoneal injection (100 μg/kg) using real-time PCR method. We found significant changes in the expression of 34 genes involved in inflammation processes. The detailed analysis of quantitative data showed that the Bcl6 gene, which plays a main role in the formation and development of the immune system, exhibited significant changes in its expression levels in response to injection of each of the peptides. Also, we observed expression changes for Bcl6 target and corepressor genes under the influence of Selank and its fragments. Our results showed that Selank and its fragments caused a number of alterations in the expression of genes involved in inflammation. The data obtained confirmed the participation of Selank in the processes of regulation of inflammation in the body. The complex biological effect of Selank may be partially determined by the systematic effect of this peptide on genomic expression.
先前的研究表明，合成的促吞噬素类似物Selank会在大鼠海马体和脾细胞中引发转录组反应，并可能参与机体炎症过程的调节。在这项研究中，我们研究了Selank及其两个片段对炎症相关基因表达的影响。我们使用实时PCR方法分析了在Selank单次腹腔注射（100μg/kg）后6小时和24小时，小鼠脾脏中84个参与炎症过程的基因（如趋化因子、细胞因子及其受体）的表达。我们发现34个参与炎症过程的基因的表达有显著变化。对定量数据的详细分析表明，在免疫系统的形成和发育中起主要作用的Bcl6基因，在注射每种肽后其表达水平都有显著变化。此外，我们还观察到在Selank及其片段的影响下，Bcl6靶基因和共抑制因子基因的表达发生了变化。我们的结果表明，Selank及其片段导致了许多参与炎症的基因表达发生改变。获得的数据证实了Selank参与了机体炎症调节过程。Selank的复杂生物学效应可能部分由该肽对基因组表达的系统性作用所决定。
