Schweiker Katrina L, Makhatadze George I
Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, USA.
Methods Enzymol. 2009;466:527-47. doi: 10.1016/S0076-6879(09)66022-5. Epub 2009 Nov 13.
Pressure perturbation calorimetry (PPC) is a new technique that makes possible to study the volumetric changes that occur upon protein unfolding. Here, we summarize the thermodynamic foundation of the method and introduce a two-state model for the analysis of the unfolding data monitored by PPC. Several examples of data analysis illustrating potential pitfalls and solutions are discussed.
压力扰动量热法（PPC）是一种新技术，它能够研究蛋白质展开过程中发生的体积变化。在此，我们总结该方法的热力学基础，并介绍一种用于分析由PPC监测的展开数据的双态模型。讨论了几个说明潜在陷阱和解决方案的数据分析示例。
