从感染牛白血病病毒的牛血清中分离肿瘤相关抗原。

Isolation of tumor-associated antigen from sera of bovine leukemia virus-infected cattle.

作者信息

Kitamura R, Onuma M, Kawakami K, Kirisawa R, Kawakami Y

机构信息

Department of Veterinary Microbiology, Rakuno Gakuen University, Hokkaido.

出版信息

Microbiol Immunol. 1990;34(2):163-70. doi: 10.1111/j.1348-0421.1990.tb01001.x.

DOI:10.1111/j.1348-0421.1990.tb01001.x

PMID:2161072

Abstract

Tumor-associated antigens (TAA) expressed on the surface of enzootic bovine leukemia (EBL) cells were detected and separated from sera of bovine leukemia virus (BLV)-positive cattle using monoclonal antibody-conjugated immunoaffinity matrix. Eluted fraction from these sera showed 3 polypeptides with molecular weights of 70K, 52K, and 30K daltons, and these polypeptides reacted with a monoclonal antibody against TAA. However, only 70K peptide was isolated from culture supernatant of EBL B-cell line. We also tried to examine a reversed passive hemagglutination test to develop a rapid screening system of serum TAA level, but its sensitivity was below the level of detection when EBL sera was applied directly. This is the first report on the existence of tumor antigens in sera from leukemic cattle.

摘要

利用单克隆抗体偶联免疫亲和基质，检测并分离了地方性牛白血病（EBL）细胞表面表达的肿瘤相关抗原（TAA），这些抗原来自牛白血病病毒（BLV）阳性牛的血清。这些血清的洗脱组分显示出3种分子量分别为70K、52K和30K道尔顿的多肽，并且这些多肽与抗TAA的单克隆抗体发生反应。然而，仅从EBL B细胞系的培养上清液中分离出了70K的肽。我们还尝试检测反向被动血凝试验，以开发一种血清TAA水平的快速筛选系统，但直接应用EBL血清时，其灵敏度低于检测水平。这是关于白血病牛血清中存在肿瘤抗原的首次报道。