Onuma M
Department of Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Japan.
Vet Immunol Immunopathol. 1989 Oct;22(3):213-21. doi: 10.1016/0165-2427(89)90008-1.
Specific tumor-associated antigen (TAA) was detected on enzootic bovine leukosis (EBL) cells by monoclonal antibodies against TAA. One of the monoclonal antibodies, c143, reacted with all EBL tumor cells tested but not with bovine leukemia virus (BLV) antigens. c143 reacted slightly with bovine fetal thymus and mitogen-stimulated lymphocytes from BLV-free cows but not with normal bovine lymphoid cells. TAA may be a good tumor marker of EBL tumor cells. We sacrificed eight TAA-positive but clinically normal animals and examined them in order to elucidate whether or not they had gross or histological tumors. At necropsy, four animals had tumors macroscopically. Three animals had no tumors histologically but had initial lesions showing follicular hyperplasia and the TAA on affected lymph nodes. The one remaining showed medullary hyperplasia in the spleen but there were no findings of tumors. Thus, c143 is a useful tool not only for diagnosing EBL, but also for screening of BLV-infected cattle with potential to develop tumors in the future.
通过针对肿瘤相关抗原（TAA）的单克隆抗体，在地方流行性牛白血病（EBL）细胞上检测到了特异性肿瘤相关抗原。其中一种单克隆抗体c143与所有测试的EBL肿瘤细胞发生反应，但不与牛白血病病毒（BLV）抗原发生反应。c143与牛胎儿胸腺以及来自无BLV奶牛的有丝分裂原刺激的淋巴细胞有轻微反应，但与正常牛淋巴细胞无反应。TAA可能是EBL肿瘤细胞的良好肿瘤标志物。我们处死了8只TAA呈阳性但临床正常的动物，并对其进行检查，以阐明它们是否有肉眼可见或组织学上的肿瘤。尸检时，4只动物肉眼可见有肿瘤。3只动物组织学上没有肿瘤，但有显示滤泡增生的初始病变以及受影响淋巴结上的TAA。剩下的1只动物脾脏出现髓样增生，但未发现肿瘤迹象。因此，c143不仅是诊断EBL的有用工具，也是筛查未来有可能发生肿瘤的BLV感染牛的有用工具。
