College of Material Science and Opto-Electronic Technology, Graduate University of Chinese Academy of Sciences, Beijing, China.
Bioresour Technol. 2011 Aug;102(15):7349-53. doi: 10.1016/j.biortech.2011.04.096. Epub 2011 May 6.
Methanol was produced from methane with a high conversion rate using a high cell density process with Methylosinus trichosporium OB3b in the presence of a high concentration of phosphate buffer. More than 1.1 g/L methanol accumulated in the reaction media under optimized reaction conditions (17 g dry cell/L, 400 mmol/L phosphate, and 10 mmol/L MgCl(2)) in the presence of 20 mmol/L sodium formate. The conversion rate of methane was over 60%. About 0.95 g/L methanol was produced when the biotransformation was carried out in a membrane aerated reactor into which methane and oxygen were introduced via two separate dense silicone tubing. Our results provide an efficient method and a promising process for high-rate conversion of methane to methanol.
甲醇可以利用甲烷在高细胞密度下经嗜甲基菌（Methylosinus trichosporium OB3b）转化，同时在高浓度磷酸盐缓冲液中高效合成，转化率超过 60%。在优化反应条件（17 g 干细胞/L、400 mmol/L 磷酸盐和 10 mmol/L MgCl(2))下，当存在 20 mmol/L 甲酸钠时，反应介质中可积累超过 1.1 g/L 的甲醇。当生物转化在膜曝气反应器中进行时，甲烷和氧气通过两条单独的密集硅树脂管引入，可生产约 0.95 g/L 的甲醇。我们的研究结果为高效、快速地将甲烷转化为甲醇提供了一种有前景的方法。
