Department of Crop and Soil Science, Oregon State University, 3017 Agricultural Life Science Building, Corvallis, OR, 97331, USA.
Department of Chemical Biological and Environmental Engineering, Oregon State University, Corvallis, OR, USA.
J Ind Microbiol Biotechnol. 2018 Mar;45(3):201-211. doi: 10.1007/s10295-018-2010-z. Epub 2018 Jan 19.
Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b's methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.
由于北美天然气的繁荣，人们对从甲烷生产其他化学产品重新产生了兴趣。我们研究了将专性甲烷营养菌 Methylosinus trichosporium OB3b 固定在海藻酸盐珠中的可行性，并通过环丙烷选择性失活甲醇脱氢酶 (MDH) 以生产甲醇。在分批培养和半连续流动柱中，暴露于环丙烷或环丙醇的海藻酸盐固定化细胞导致 MDH 活性的大部分丧失（>80%），从而使甲醇积累到显著浓度，同时保留 M. trichosporium OB3b 的甲烷单加氧酶能力。此后，由于大部分 MDH 活性的恢复，甲醇生产的效率下降；然而，随后的抑制期导致甲醇生产效率的重新提高，固定化细胞保留甲烷氧化活性至少 14 天。
