Institute of Biomedical Technology, University of Tampere, Finland.
J R Soc Interface. 2011 Dec 7;8(65):1736-47. doi: 10.1098/rsif.2011.0211. Epub 2011 May 25.
Mechanical stimulation is an essential factor affecting the metabolism of bone cells and their precursors. We hypothesized that vibration loading would stimulate differentiation of human adipose stem cells (hASCs) towards bone-forming cells and simultaneously inhibit differentiation towards fat tissue. We developed a vibration-loading device that produces 3g peak acceleration at frequencies of 50 and 100 Hz to cells cultured on well plates. hASCs were cultured using either basal medium (BM), osteogenic medium (OM) or adipogenic medium (AM), and subjected to vibration loading for 3 h d(-1) for 1, 7 and 14 day. Osteogenesis, i.e. differentiation of hASCs towards bone-forming cells, was analysed using markers such as alkaline phosphatase (ALP) activity, collagen production and mineralization. Both 50 and 100 Hz vibration frequencies induced significantly increased ALP activity and collagen production of hASCs compared with the static control at 14 day in OM. A similar trend was detected for mineralization, but the increase was not statistically significant. Furthermore, vibration loading inhibited adipocyte differentiation of hASCs. Vibration did not affect cell number or viability. These findings suggest that osteogenic culture conditions amplify the stimulatory effect of vibration loading on differentiation of hASCs towards bone-forming cells.
机械刺激是影响骨细胞及其前体细胞代谢的一个重要因素。我们假设，振动负荷会刺激人脂肪干细胞（hASCs）向成骨细胞分化，同时抑制向脂肪组织分化。我们开发了一种振动加载装置，可在 50 和 100 Hz 的频率下产生 3g 的峰值加速度，用于培养在微孔板上的细胞。hASCs 分别在基础培养基（BM）、成骨培养基（OM）或成脂培养基（AM）中培养，并在 3 h/d 的条件下接受振动加载，持续 1、7 和 14 天。使用碱性磷酸酶（ALP）活性、胶原蛋白产生和矿化等标志物分析成骨作用，即 hASCs 向成骨细胞的分化。与 OM 中的静态对照组相比，50 和 100 Hz 的振动频率在 14 天时均显著增加了 hASCs 的 ALP 活性和胶原蛋白产生。矿化也呈现出类似的趋势，但增加并不具有统计学意义。此外，振动加载抑制了 hASCs 的脂肪细胞分化。振动不会影响细胞数量或活力。这些发现表明，成骨培养条件放大了振动加载对 hASCs 向成骨细胞分化的刺激作用。
