Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
Kushiro City General Hospital, Kushiro, Japan.
J Gen Virol. 2011 Sep;92(Pt 9):2201-2208. doi: 10.1099/vir.0.033001-0. Epub 2011 May 25.
Although G2P[4] rotaviruses are common causes of acute childhood diarrhoea in Africa, to date there are no reports on whole genomic analysis of African G2P[4] strains. In this study, the nearly complete genome sequences of two Kenyan G2P[4] strains, AK26 and D205, detected in 1982 and 1989, respectively, were analysed. Strain D205 exhibited a DS-1-like genotype constellation, whilst strain AK26 appeared to be an intergenogroup reassortant with a Wa-like NSP2 genotype on the DS-1-like genotype constellation. The VP2-4, VP6-7, NSP1, NSP3 and NSP5 genes of strain AK26 and the VP2, VP4, VP7 and NSP1-5 genes of strain D205 were closely related to those of the prototype or other human G2P[4] strains. In contrast, their remaining genes were distantly related, and, except for NSP2 of AK26, appeared to originate from or share a common origin with rotavirus genes of artiodactyl (ruminant and camelid) origin. These observations highlight the complex evolutionary dynamics of African G2P[4] rotaviruses.
虽然 G2P[4]轮状病毒是非洲儿童急性腹泻的常见病因，但迄今为止，尚无关于非洲 G2P[4] 株全基因组分析的报道。在本研究中，分析了分别于 1982 年和 1989 年检测到的两株肯尼亚 G2P[4] 株 AK26 和 D205 的近乎完整的基因组序列。株 D205 表现出 DS-1 样基因型组合，而株 AK26 似乎是一种基因间重组体，在 DS-1 样基因型组合上具有 Wa 样 NSP2 基因型。株 AK26 的 VP2-4、VP6-7、NSP1、NSP3 和 NSP5 基因和株 D205 的 VP2、VP4、VP7 和 NSP1-5 基因与原型或其他人类 G2P[4] 株密切相关。相比之下，它们的其余基因则远缘相关，除了 AK26 的 NSP2 外，似乎起源于或与偶蹄目（反刍动物和骆驼科动物）来源的轮状病毒基因具有共同的起源。这些观察结果突出了非洲 G2P[4] 轮状病毒的复杂进化动态。
