Laboratory of Molecular Epidemiology of Viral Infections, I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, 71 Malaya Yamskaya Str., Nizhny Novgorod, Russia, 603950.
Laboratory of Metagenomics and Molecular Indication of Pathogens, I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, 71 Malaya Yamskaya Str., Nizhny Novgorod, Russia, 603950.
Virus Genes. 2020 Oct;56(5):537-545. doi: 10.1007/s11262-020-01771-3. Epub 2020 May 29.
Rotavirus A is a dynamically evolving pathogen causing acute gastroenteritis in children during the first years of life. In the present study, we conducted a phylodynamic analysis based on the complete sequences of 11 segments of rotaviruses with the G4P[8] and G2P[4] genotypes isolated in Russia in 2017. Since rotavirus has a segmented genome, our analysis was performed using the Bayesian approach based on separate samples of nucleotide sequences for each gene of the strains studied. For the strain with the genotype G4P[8], the most likely geographical locations of the nearest common ancestor were Russia (VP7, VP4, VP6), China (VP1), Thailand (VP3), Belgium (NSP1), Hungary (VP2, NSP2, NSP3), Italy (NSP4) and Japan (NSP5). For the strain with the G2P[4] genotype, India (VP7, VP4, VP6, NSP1, NSP4), Malawi (VP2, NSP2, NSP3), Australia (VP1), Italy (NSP5) and Bangladesh (VP3). The closest common ancestor of the strain with the genotype G4P[8] circulated in 2001-2012, depending on the gene being analyzed. For the strain with the G2P[4] genotype, the closest common ancestor dates from 2006 to 2013.
轮状病毒 A 是一种具有动态进化能力的病原体，可导致生命最初几年的儿童急性肠胃炎。在本研究中，我们对 2017 年在俄罗斯分离的 G4P[8]和 G2P[4]基因型的 11 段轮状病毒完整序列进行了系统发育分析。由于轮状病毒具有分段基因组，因此我们的分析是针对研究株的每个基因的核苷酸序列单独样本使用贝叶斯方法进行的。对于基因型为 G4P[8]的菌株，最有可能的最近共同祖先的地理位置是俄罗斯（VP7、VP4、VP6）、中国（VP1）、泰国（VP3）、比利时（NSP1）、匈牙利（VP2、NSP2、NSP3）、意大利（NSP4）和日本（NSP5）。对于基因型为 G2P[4]的菌株，最有可能的最近共同祖先的地理位置是印度（VP7、VP4、VP6、NSP1、NSP4）、马拉维（VP2、NSP2、NSP3）、澳大利亚（VP1）、意大利（NSP5）和孟加拉国（VP3）。基因型为 G4P[8]的菌株的最近共同祖先在 2001-2012 年之间循环，具体取决于分析的基因。对于基因型为 G2P[4]的菌株，最近共同祖先可追溯到 2006 年至 2013 年。
