促肾上腺皮质激素释放因子、尾加压素I和蛙皮素刺激金鱼神经中间叶细胞释放源自阿片-促黑素细胞皮质素原的肽。

CRF, urotensin I, and sauvagine stimulate the release of POMC-derived peptides from goldfish neurointermediate lobe cells.

作者信息

Tran T N, Fryer J N, Lederis K, Vaudry H

机构信息

Department of Anatomy, Faculty of Medicine, University of Ottawa, Ontario, Canada.

出版信息

Gen Comp Endocrinol. 1990 Jun;78(3):351-60. doi: 10.1016/0016-6480(90)90025-h.

DOI:10.1016/0016-6480(90)90025-h

PMID:2161377

Abstract

In teleost fishes, the melanotropes of the neurointermediate lobe of the pituitary gland release numerous peptides--adrenocorticotropin (ACTH), melanotropin (MSH), lipotropin (LPH), corticotropin-like intermediate lobe peptide (CLIP), and endorphin--which are derived from the precursor molecule proopiomelanocortin. Superfused, isolated, dispersed goldfish neurointermediate lobe cell columns were used to investigate the release of immunoreactive (ir) alpha-MSH and ir ACTH from goldfish melanotropes. Stimulation of neurointermediate lobe cell columns with pulses of the structurally homologous peptides, Catostomus urotensin I (UI), ovine corticotropin-releasing factor (oCRF), or sauvagine, produced a significant increase in the concomitant release of ir alpha-MSH and ir ACTH. UI was two to three times as potent as ovine CRF or sauvagine. These studies suggest that CRF- and UI-like peptides stimulate the secretory activity of teleost melanotropes.

摘要

在硬骨鱼类中，脑垂体神经中间叶的促黑素细胞释放多种肽类物质——促肾上腺皮质激素（ACTH）、促黑素（MSH）、促脂解素（LPH）、促肾上腺皮质激素样中间叶肽（CLIP）和内啡肽——这些肽类均来源于前体分子阿黑皮素原。利用经体外灌流的、分离的、分散的金鱼神经中间叶细胞柱来研究金鱼促黑素细胞释放免疫反应性（ir）α-MSH和ir ACTH的情况。用结构同源肽类，如胭脂鱼尾加压素I（UI）、羊促肾上腺皮质激素释放因子（oCRF）或蛙皮素的脉冲刺激神经中间叶细胞柱，会使irα-MSH和ir ACTH的伴随释放显著增加。UI的效力是羊CRF或蛙皮素的两到三倍。这些研究表明，CRF样肽和UI样肽可刺激硬骨鱼类促黑素细胞的分泌活性。