Koroleva G A, Gracheva L A, Voroshilova M K
Vopr Virusol. 1978 Sep-Oct(5):611-8.
Virological examinations of 72 specimens obtained during an outbreak of a poliomyelitis-like disease in Bulgaria, 1975, yielded 23 strains of enterovirus type 71 (E71) and 5 strains of enteroviruses of other types. The viruses were isolated in green monkey kidney cell cultures, in newborn cotton rats, newborn white mice and in monkeys. Most of E71 strains (16 out of 23) were isolated from the autopsy materials (brains and spinal cords) from 9 children dying of poliomyelitis, meningoencephalitis, and encephalitis. The remaining E71 strains and all the strains of other enterovirus serotypes, with the exception of ECHO7, were isolated from stool specimens of children with aseptic meningitis and their contacts. The antigenic similarity of the group of Bulgarian strains and their relationship with the prototype BrCr strain of E71 was established. The conclusion is that E71 was the causative agent of an outbreak of a poliomyelitis-like disease in Bulgaria.
1975年在保加利亚一次脊髓灰质炎样疾病暴发期间采集的72份标本进行病毒学检测，分离出23株71型肠道病毒（E71）和5株其他型肠道病毒。这些病毒在绿猴肾细胞培养物、新生棉鼠、新生小白鼠和猴子体内分离得到。大多数E71毒株（23株中的16株）是从9名死于脊髓灰质炎、脑膜脑炎和脑炎的儿童尸检材料（脑和脊髓）中分离出来的。其余的E71毒株以及除埃可病毒7型（ECHO7）外的所有其他肠道病毒血清型毒株，均从患无菌性脑膜炎儿童及其接触者的粪便标本中分离得到。确定了保加利亚毒株组的抗原相似性及其与E71原型BrCr毒株的关系。结论是，E71是保加利亚一次脊髓灰质炎样疾病暴发的病原体。
